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Vanadyl sulfate suppresses the proliferation of MCF-7 breast cancer cells by inducing G2/M phase arrest and caspase-dependent apoptosis.
We previously reported that vanadyl sulfate (VOSO(4)) was a potent anti-proliferative agent in human lymphocytic leukemia cells. Here, we examined the anti-proliferative effects of VOSO(4) on human MCF-7 breast cancer cells. VOSO(4) inhibited the proliferation of MCF-7 cells with an IC(50) value of approximately 4.6 microM. The cells were arrested at G(2)/M phase with a G(2)/M phase accumulation at 5.9-fold increase in MCF-7 cells treated with VOSO(4) (10 microM) for 24 h. VOSO(4) treatment also induced caspase-3-like activity and poly-ADP-ribose polymerase cleavage in MCF-7 cells, suggesting that the VOSO(4)-induced apoptosis occurred caspase-dependent. As expected, these effects were accompanied by the induction of G(2)/M phase cell cycle arrest and down-regulation of the expression of anti-apoptotic proteins (BCL-2, BCL-XL, and MCL-1) and pro-apoptotic proteins (Bax, Bim, and Bim-EL). These results suggested that VOSO(4) may be useful in the development of new therapeutic agents for the treatment of breast cancer.

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